Khatam Kari is one of the traditional Iranian arts with a long history, and numerous works of this art have survived from past eras. Considering the limited technical studies on these works, their study plays an important role in better understanding the process of making Khatam in the past. The diversity of Khatam materials and their different reactions to conservation methods have a great impact on adopting the optimal conservation and treatment method. As a result, identifying their constituent materials will be necessary to choose the correct method of dealing with this type of works. Fourier transform infrared spectroscopy (FTIR) is one of the methods that can be used to identify the nature of the organic materials that make up the khatam. Therefore, in this study, the khatam door khatam of the Seyyed Mosque of Isfahan from the Qajar period was examined as a case study. For this purpose, FTIR spectroscopy was used to investigate the structure and identify the materials used in its construction. The results showed that the red-brown and black pieces in this khatam sample are made of wood and the white pieces are made of bone. Also, Animal Glue was used as an adhesive in wrapping the khatam and lining it, and a mixture containing terpenoid resins was used to finish and coat the khatam .
