Cleaning and washing of historical artifacts and textiles remain one of the most critical and challenging aspects of conservation and restoration. To evaluate the quality of detergents used in the conservation of historical textiles, laboratory samples are required. However, due to the unique characteristics of historical artifacts, existing standardized soiling methods are insufficient for researchers in this field. Therefore, this study aims to prepare artificially soiled cotton fabric samples subjected to accelerated aging. To achieve this goal, the type and amount of soiling were first determined, followed by monitoring and analyzing the degradation of samples during accelerated aging. The research methodology was based on analytical-comparative approaches, with data collected through experiments such as Fourier Transform Infrared (FTIR) spectroscopy, tensile strength testing, equilibrium moisture content, electrical conductivity, color measurement, and pH assessment. The procedure involved preparing, soiling, and aging cotton fabrics, with degradation changes monitored and compared to those of clean samples. Results indicate that after approximately 700 hours of accelerated aging under elevated temperature and humidity (110°C and 90% relative humidity), the samples reached a relatively stable state in terms of degradation changes. This stability allows minor adverse effects of detergents to be clearly detectable. Thus, the minimum time required for aging and preparing laboratory samples of soiled fabrics is approximately 700 hours at 110°C and 90% relative humidity.